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金属抗菌肽SIF_4对大肠杆菌的抑菌机制

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成果类型:
期刊论文
论文标题(英文):
Antimicrobial mechanism of metal antimicrobial peptide SIF_4 against Escherichia coli
作者:
肖怀秋;李玉珍;林亲录;赵谋明;周全;...
通讯作者:
Li, Y.
作者机构:
[周全; 李玉珍; 赵一纯] School of Pharmaceutical and Bioengineering, Hunan Chemical Vocational Technology College, Zhuzhou, 412000, China
[林亲录] College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, China
[赵谋明] College of Food Science and Engineering, South China University of Technology, Guangzhou, 510000, China
[肖怀秋] School of Pharmaceutical and Bioengineering, Hunan Chemical Vocational Technology College, Zhuzhou, 412000, China, College of Food Science and Engineering, South China University of Technology, Guangzhou, 510000, China
通讯机构:
[Li, Y.] S
School of Pharmaceutical and Bioengineering, China
语种:
中文
关键词:
金属抗菌肽;大肠杆菌;细胞壁通透性;细胞膜损伤
关键词(英文):
cell membrane damage;cell wall permeability;Escherichia coli;metal antimicrobial peptide
期刊:
食品与发酵工业
ISSN:
0253-990X
年:
2022
卷:
48
期:
1
页码:
111-116
基金类别:
2021JJ60040:湖南省自然科学基金科教联合基金项目 20C0686:湖南省教育厅科学研究项目
机构署名:
本校为其他机构
院系归属:
食品科学与工程学院
摘要:
大肠杆菌为低感染剂量肠道致病病原体,可引起严重的食品公共卫生问题。该研究从细胞壁通透性、胞内K~+和生物大分子泄露、细胞表面疏水性、细胞膜通透性和表面zeta电位等方面系统研究了金属抗菌肽SIF_4对大肠杆菌的抑菌机制。研究发现,SIF_4的最小抑菌质量浓度为0.4 mg/L;SIF_4处理后菌体稳定期与衰亡期提前;细胞壁通透性与SIF_4浓度及温育时间呈正相关,温育1 h后,组别间细胞壁通透性有显著差异(P < 0.05);胞内K~+泄露随SIF_4浓度升高和温育时间延长呈递增趋势;胞内生物大分子泄露随SIF_4浓度升高与温育时间延长呈增加趋势,温育2 h后,试验组与对照组有显著差异(P < 0.05);表面疏水性随SIF_4浓度...
摘要(英文):
The antimicrobial mechanism of SIF4 against Escherichia coli from cell wall permeability (CWP), intracellular K + and bi-omacromolecule leakage, cell surface hydrophobicity (CSH), cell membrane permeability (CMP) and cell surface zeta potential (CSZP) were investigated. Results showed that minimum inhibition concentration of SIF4 was 0. 4 mg / L. The stationary phase and decline phase of E. coli came early after treated by SIF4 . CWP had a positively correlation with the SIF4 concentration and incubation time, after 1. 0 h incubation, there was...

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