期刊论文

中文

食品与发酵工业

0253-990X

2014

40

5

87-92

10.13995/j.cnki.11-1802/ts.2014.05.021

湖南省中药学重点学科(湘教通[2011]76号) 湖南中医药大学生物工程重点学科(校行科[2012]2号)共同资助

本校为其他机构

采用国产SepliteLX-l000EP树脂对青霉菌柚苷酶进行固定化,以期提高其稳定性和利用效率。将单因素试验和Box-Behnken试验设计结合对影响Seplite LX-1000EP树脂固定化柚苷酶的主要影响因素进行分析、优化。结果显示,最佳固定化条件为:酶液pH 6.5、固定化温度为26.6℃、载体投放量为0.79 g、固定化时间为24 h,此时固定化柚苷酶酶活达到3 949.32 U/g,酶活回收率提高至44. 79%, 蛋白固定率为98.43%, 说明将Seplite LX-1000EP树脂用于柚苷酶的固定化是切实可行的。

In order to improve naringinase catalytic performance,covalent immobilization of naringinase using domestic Seplite LX-1000EP resin was studied. Monofactorial experiment and Box-Behnken design were adopted to optimize naringinase immobilization conditions,then the data was analyzed with SAS software version 8. 0. The optimum immobilization conditions were summed up: pH of immobilization system,immobilization temperature,the ration of carrier addition,and immobilized time were 6. 5,26. 6℃,0. 79 g Seplite LX-1000EP resin per 5 mL enzyme solution...