期刊论文

Function of Vacuolar Protein Sorting CfVps26 in Colletotrichum fructicola on Camellia oleifera

中文

林业科学

1001-7488

2021

57

8

94-101

10.11707/j.1001-7488.20210809

国家自然科学基金项目（32071765）；

本校为第一机构

【目的】研究液泡分选蛋白Vps26在油茶果生刺盘孢中的生物学功能,为阐明Vps26的作用机制和防治油茶炭疽病提供理论依据。【方法】采用Over-lap法构建CfVPS26基因敲除载体片段,利用PEG介导的转化法将该片段转入果生刺盘孢原生质体中,对具有潮霉素抗性的转化子进行验证筛选; PCR扩增启动子在内的CfVPS26基因回补片段,构建回补质粒并转入突变体原生质体中,荧光筛选回补菌株;对野生型菌株、突变体和回补菌株进行营养生长、附着胞形成率、细胞壁胁迫、糖原染色及致病力等生物学表型测定。【结果】筛选验证获得CfVPS26基因缺失突变体ΔCfvps26;该突变体生长速率均显著小于野生型菌株和回补菌株,气生菌...

【Objective】Colletotrichum fructicola is a major epidemic and causes anthracnose of Camellia oleifera.This paper aims to study the biological functions of the vacuolar protein sorting Vps26 in C.fructicola to provide a theoretical basis for the prevention and control of oil-tea tree anthracnose,and also for further analysis of Vps26 action mechanism.【Method】The over-lap method was used to construct the CfVPS26 gene knockout vector fragment which was transformed into the protoplast of C.fructicola by using PEG-mediated transformation method t...