期刊论文

Zhou, Guoying

中文

林业科学

1001-7488

2016

52

10

80-88

10.11707/j.1001-7488.20161010

31570641:国家自然科学基金 31100479:国家自然科学基金

本校为第一且通讯机构

【目的】研究海南省油茶树及其他寄主植物炭疽病菌果生刺盘孢菌群体遗传结构、群体遗传多样性及单倍型地理分布,探索海南地区不同寄主植物炭疽病菌的分子遗传变异规律。【方法】利用单孢分离法对采集自海南省南圣镇、通什镇、枫木林场和澄迈林场4个地区9种不同寄主植物的炭疽病病叶进行炭疽菌分离,并对每个菌株ITS,CAL和GAPDH 3个基因进行PCR扩增和测序,按顺序对3个基因的序列进行拼接,然后采用GenALEx6, DnaSP 5.0,TCS1.21和MEGA6.0软件分别对数据进行分析。【结果】分离、鉴定共获得12个病菌种群的108个果生刺盘孢菌病菌样品,可推导出18种单倍体型(haplotype), 其中单倍型Haplotype11共70个菌...

Objective: The population genetic structure genetic diversity and the geographical distribution of haplotype patterns of Colletotrichum fructicola from Oil-tea and other host plants in Hainan province were analysised to explore the molecular genetic variation pattern of the pathogen in different hosts.Method: C.fructicola associated with anthracnose diseases of oil-tea and other native plants were isolated using single spore isolation and confirmed by DNA sequencing of the partial sequences of rDNA-ITS, CAL and GAPDH region. GenALEx6, DnaSP 5.0...