期刊论文

中文

林业科学

1001-7488

2015

51

3

41-48

10.11707/j.1001-7488.20150306

(201204403):国家林业公益性行业科研专项重大项目 (2013RJ003):中南林业科技大学引进***人才科研启动基金

本校为第一机构

【目的】油桐种仁中产出的桐油经济利用价值极高。桐油中α－桐酸的含量高达70%，然而植物体内α－桐酸代谢通路的研究还未见报道，这对直接筛选油桐α－桐酸代谢通路相关酶基因造成一定的困难。α－亚麻酸作为α－桐酸的同分异构体，其代谢通路的研究则较为深入，能为α－桐酸代谢通路的解析提供参考。因此，本研究期望在油桐种仁转录组数据的基础上，解析油桐的α－亚麻酸代谢途径，为油桐α－桐酸代谢机理的阐明提供理论参考。此外，通过调控这些基因的表达模式以及开发与之紧密连锁的分子标记，可大大加快油桐遗传改良和分子育种的进程。【方法】采用 RNA-Seq技术对油桐种仁3个不同油脂合成...

Objective]Tung oil extracted from tung tree ( Vernicia fordii) kernels has extremely high economic value. Tung oil contains α-eleostearic acid up to 70 percent. However,there are some difficulties in screening enzyme genes involved in α-eleostearic acid metabolism of tung tree due to very few studies being carried out on plant α-eleostearic acid metabolism pathway. Studies on metabolism pathway of α-linolenic acid,the isomers of α-eleostearic acid,were more in-depth and provide an example for analyses of metabolism pathway ofα-eleostearic...