为优化低值豆粕液态发酵生产大豆多肽工艺,应用Minimum Run Equireplicated Res IV析因设计进行了主效因子的筛选,根据主效因子影响及变化方向进行爬陡坡试验,最后,应用二次旋转中心复合响应面设计对液态发酵多肽工艺进行了优化,优化工艺条件为豆粕浓度6.0%、pH 8.0、装瓶量93.0 mL.300 mL~(-1)。最优条件下模型预测多肽含量为707.204μg.mL~(-1),验证试验结果为683.023±9.23μg.mL~(-1)(n=6)。
摘要(英文)：
To investigate the optimal producing conditions of soybean peptide（SBP）from soybean meal（SBM）by liquid state fermentation（LSF）,Minimum Run Equireplicated Res IV design was used to screen main factors affecting SBP production,steepest ascent experiment was adopted to approach the optimal region based the results from the factorial design.At last,quadratic rotating central composite design（QRCCD）was applied to optimize the SBP producing parameters.The optimal condition for SBP biosynthesis are 6.0% of SBM,pH 8.0 and media amount is 93.0 m...
