Dopamine-supported HPLC post-column derivatization to fluorescence: Simultaneous and sensitive detection of eight tea polyphenols

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成果类型：

期刊论文

作者：

Li, Hongchen;Cheng, Jing;Ge, Hanbing;Sun, Jingbo;Chen, Zihan;...

通讯作者：

Dong Xu<&wdkj&>Zhiqin Yuan

作者机构：

[Li, Hongchen; Chen, Zihan; Ren, Jiali; Sun, Jingbo] National Engineering Laboratory for Rice and By-products Further Processing, College of Food Science and Engineering, Central South University of Forestry & Technology, Changsha 410004, China

[Cheng, Jing] Technology Center of Changsha Customs, Hunan Academy of Inspection and Quarantine, Changsha 410004, China

[Ge, Hanbing] College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China

[Du, Yi] Analysis Center, Department of Chemistry, Tsinghua University, Beijing 100084, China

[Xu, Dong] National Engineering Laboratory for Rice and By-products Further Processing, College of Food Science and Engineering, Central South University of Forestry & Technology, Changsha 410004, China. Electronic address: philip198349@gmail.com

通讯机构：

[Dong Xu] N

[Zhiqin Yuan] C

College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China<&wdkj&>National Engineering Laboratory for Rice and By-products Further Processing, College of Food Science and Engineering, Central South University of Forestry & Technology, Changsha 410004, China

语种：

英文

关键词：

Azamonardine;Derivatization;Fluorescence detection;High throughput analysis;Tea sample

期刊：

Food Chemistry

ISSN：

0308-8146

年：

2025

卷：

464

期：

Pt 1

页码：

141582

DOI：

10.1016/j.foodchem.2024.141582

基金类别：

CRediT authorship contribution statement Hongchen Li: Software, Methodology, Investigation, Data curation. Jing Cheng: Validation, Data curation. Hanbing Ge: Validation, Software, Methodology, Investigation. Jingbo Sun: Validation, Software, Investigation. Zihan Chen: Writing – original draft, Software. Jiali Ren: Visualization, Validation. Yi Du: Writing – original draft, Validation. Dong Xu: Writing – review & editing, Writing – original draft, Supervision, acquisition, Conceptualization. Zhiqin Yuan: Writing – review &

机构署名：

本校为第一且通讯机构

院系归属：

食品科学与工程学院

摘要：

The effective differentiation and detection of multiple tea polyphenols are often challenging due to their subtle structural similarities. Although post-column derivatization HPLC strategies are commonly employed to distinguish multiple targets, the short physical distance between chromatographic column and detector limits reaction time, thereby reducing the derivatization efficiency. Dopamine (DA) reacts rapidly with resorcinol to form fluorescent azamonardine products, making fast fluorometric derivatization of tea polyphenols containing resorcinol motifs possible. In this study, a DA-driven...

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Dopamine-supported HPLC post-column derivatization to fluorescence: Simultaneous and sensitive detection of eight tea polyphenols

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