Zelkova schneideriana Hand.-Mazz. is a high-value plant used for hardwood tumber production in China. Because of over-harvesting and limited numbers of seedlings for plantations, Z. schneideriana has become an endangered species in China. To grow this plant sustainably, we generated a protocol to reproduce this species using tissue culture. Leaf explants were cultured on Wood Plant Medium (WPM), Murashige and Skoog (MS), 1/2MS (main elements), or B5 media variously supplemented with 6-benzylaminopurine (BA), naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), or 2, 4-dichlorophenoxyacet...
