Leaf organ of Zelkova schneideriana was used as explants to induce callus, then differentiate and regenerate plantlets from callus. Plantlets regeneration technology was studied. Results showed that it was easy to induce callus from leaf organ of Zelkova schneideriana and the inducing ratio of callus could reach above 90% by using young leaves. It was difficult to differentiate and regenerate plantlets by indu cing callus. The ratio of callus differentiation was about 10%. Callus induced form ...