INTRODUCTION: Wood is primarily made up of secondary xylem cell walls, with lignin, cellulose, and hemicellulose as the main chemical components. The presence of lignin represents recalcitrance to wood pulping and biofuel conversion. Consequently, reducing lignin content is a key approach to improving wood properties and optimizing its processing. METHODS: In this study, we suppressed lignin biosynthesis by overexpressing a mutated transcription repressor PdLTF1(AA) and enhanced cellulose synthesis simultaneously by introducing cellulose synthase genes, PdCesA4, PdCesA7A, or PdCesA8A, specific...
