期刊论文

Effects of selenium supplement on atherogenesis of ApoE-knockout mice fed high fat diet

中文

中华心血管病杂志

Zhonghua xin xue guan bing za zhi

0253-3758

2016

44

3

244-249

10.3760/cma.j.issn.0253-3758.2016.03.011

国家自然科学基金(81202170)National Natural Science Foundation of China (81202170)

本校为其他机构

目的探讨补充硒元素对小鼠动脉粥样硬化的影响和对血管内皮功能的作用。方法将低硒高脂饲料饲养的载脂蛋白E基因敲除小鼠按照随机数字表法,分为对照组(未补硒,10只)、低剂量补硒组(饮用水的亚硒酸钠浓度为0.1 mg/L,10只)和高剂量补硒组(饮用水的亚硒酸钠浓度为0.2 mg/L,10只)。饲养15周后,测定各组小鼠心脏和肝脏中的硒含量、血脂水平、抗氧化功能指标[包括血清超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛水平]和血管内皮功能指标[包括血清一氧化氮(NO)和内皮素1、血管内皮生长因子(VEGF)水平和主动脉根部内皮素1和VEGF的表达水平],并采用油红O染色分析主动脉根部的动脉粥...

OBJECTIVE: To investigate the effects of selenium supplement on atherogenesis and endothelial function in ApoE-knockout mice fed high fat diet. METHODS: ApoE-knockout mice fed with selenium-deficient and high fat diet were randomly allocated into 3 groups based on random number table including control group (not supplied with sodium selenite, n=10), low dosage selenium supplement group (supplied water with 0.1 mg/L sodium selenite, n=10) and high dosage selenium supplement group (supplied water with 0.2 mg/L sodium selenite, n=10). After 15 weeks, the following values were measured: the concen...