Biochemical and molecular characterization of a novel glycerol dehydratase from Klebsiella pneumoniae 2e with high tolerance against crude glycerol impurities

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成果类型：

期刊论文

作者：

Lin, Zifeng;Xiao, Yuting;Zhang, Lu;Li, Le;Dong, Congying;...

通讯作者：

Ma, Jiangshan;Liu, GQ

作者机构：

[Liu, GQ; Ma, Jiangshan; Xiao, Yuting; Zhang, Lu; Li, Le; Liu, Gao-Qiang; Dong, Congying; Lin, Zifeng] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.

[Liu, GQ; Ma, Jiangshan; Xiao, Yuting; Zhang, Lu; Li, Le; Liu, Gao-Qiang; Dong, Congying; Lin, Zifeng] Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Peoples R China.

[Liu, GQ; Ma, Jiangshan; Xiao, Yuting; Zhang, Lu; Li, Le; Liu, Gao-Qiang; Dong, Congying; Lin, Zifeng] Microbial Variety Creat Ctr, Yuelushan Natl Lab Seed Ind, Changsha 410004, Peoples R China.

通讯机构：

[Liu, GQ ; Ma, JS] C

Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.

Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Peoples R China.

Microbial Variety Creat Ctr, Yuelushan Natl Lab Seed Ind, Changsha 410004, Peoples R China.

语种：

英文

关键词：

Glycerol dehydratase;Klebsiella pneumoniae 2e;Enzymatic activity;High tolerance;Crude glycerol impurities;Coil structure;Protein flexibility

期刊：

Biotechnology for Biofuels and Bioproducts

ISSN：

2731-3654

年：

2023

卷：

期：

页码：

1-14

DOI：

10.1186/s13068-023-02427-8

基金类别：

This work was financially supported by the National Natural Science Foundation of China (31900087), The Science and Technology Innovation Program of Hunan Province (2022RC1153), Natural Science Foundation of Hunan Province (2020JJ5974, 2021JJ31151), Program of Hunan Science and Technology Innovation Team (2021RC4063), and Outstanding Youth Project of Hunan Provincial Education Department (18B169) for financial support of this study.

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本校为第一且通讯机构

院系归属：

生命科学与技术学院

摘要：

Abstract Background The direct bioconversion of crude glycerol, a byproduct of biodiesel production, into 1,3-propanediol by microbial fermentation constitutes a remarkably promising value-added applications. However, the low activity of glycerol dehydratase, which is the key and rate-limiting enzyme in the 1,3-propanediol synthetic pathway, caused by crude glycerol impurities is one of the main factors affecting the 1,3-propanediol yield. Hence, the exploration of glycerol dehydratase resources suitable for crude glycerol bioconversion is re...

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Biochemical and molecular characterization of a novel glycerol dehydratase from Klebsiella pneumoniae 2e with high tolerance against crude glycerol impurities

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