期刊论文

Construction and Analysis on the Kinetic Model of Gibberellin Acid in Batch Fermentation

中文

过程工程学报

1009-606X

2017

17

3

605-612

10.12034/j.issn.1009-606X.216304

湖南省教育厅一般资助项目（编号:15C1418）；

本校为第一机构

采用5 L全自动发酵罐对赤霉菌进行发酵培养,分时取样,记录不同时段发酵液中菌丝浓度、残糖浓度、氨基氮浓度及赤霉素浓度;将赤霉素发酵过程分为菌丝营养生长阶段（0-64 h）与菌丝产赤霉素阶段（64-192 h）,分段建立发酵动力学模型,对赤霉素发酵培养基中初始碳源、氮源浓度及碳氮质量比（C/N）进行优化.结果表明,在考察的碳源、氮源浓度适用范围内,模型计算值与实验值吻合度较好.

The concentration changes of mycelium, residual sugar, amino nitrogen and gibberellic acid (GA3) were recorded in a 5 L automatic fermenter. Based on the analysis of fermentation process, a two-stage differential kinetic model, including mycelium growth stage (0~64 h) and GA3 production phase (64~192 h), was constructed. By using the model, the initial carbon, nitrogen concentration and carbon-nitrogen mass ratio (C/N) in the fermentation medium were optimized. Within the scope of the carbon concentration and nitrogen concentration, the calcula...