期刊论文

Preparation and characterization of uricase in uricase-catalase liposomes prepared using borate buffer

中文

南方医科大学学报

Journal of Southern Medical University

1673-4254

2015

35

2

268-271

10.3969/j.issn.1673-4254.2015.02.23

国家自然科学基金

本校为其他机构

目的研究硼酸-硼砂缓冲液制备的尿酸酶-过氧化氢酶脂质体(BUCLP)中尿酸酶的体外特性。方法采用逆向蒸发法制备BUCLP,并考察其部分理化性质特性。结果 BUCLP中尿酸酶最适温度保持在40℃,最适p H值由8.5降为8.0,而Km值也由14.207μmol/L降为13.623μmol/L;尿酸酶-过氧化氢酶(UAC)与空白纳米脂质体作用后,再与FITC结合,其荧光强度高于游离UAC与FITC结合的荧光强度,且BUCLP在280 nm处的荧光强度高于游离UAC。结论尿酸酶和过氧化氢酶联合制备成BUCLP后尿酸酶的活性增加。

OBJECTIVE: To characterize the property of uricase loaded in uricase-catalase liposomes (BUCLPs) prepared using borate buffer. METHODS: BUCLPs were prepared using reverse-phase evaporation, and the physicochemical properties of uricase in the prepared BUCLPs were examined. RESULTS: The optimal temperature of BUCLP and URI was 40 degrees celsius, their optimal pH values were 8.0 and 8.5, and their Michaelis-Menten constants were 14.207 micromol/L and 13.623 micromol/L, respectively. Fluorescence intensity of nanoliposome-loaded uricase-catalase that bound to FITC was higher than that of uricase...