会议论文

Observation on the Pollination,Fertilization and Early Embryonic Development of Camellia oleifera

中文

2013

93-100

全国油茶技术协作组第一届油茶学术交流会

全国油茶技术协作组第一届油茶学术交流会论文集

2013-12-01

浙江建德

全国油茶技术协作组<&wdkj&>国家林业局<&wdkj&>中国林科院

本校为第一机构

本试验以油茶'湘林XLC15'为试材,采用荧光显微观察、切片荧光观察和石蜡切片法,研究了油茶的授粉受精和早期胚胎发育过程.结果如下:油茶授粉后2h花粉萌发,48 h花粉管长至花柱基部,60 h花粉管通过一个退化的助细胞开始进入胚囊,为珠孔受精方式,观察到的花粉管进入胚囊的比例为23％.精细胞与二极核相互融合主要发生于授粉后3～10d,精卵细胞的融合主要发生于授粉后5～16d,配子的融合属有丝分裂前类型.初生胚乳核形成后即开始进行有丝分裂,胚乳的发育类型为核型胚乳,胚乳核极不发达,胚乳细胞大、个数少.合子经历约4个月的休眠后开始发育,授粉后200 d形成球形胚,具有明显的胚柄,胚的发育类型为茄型.

　　The processes of pollination,fertilization and embryo development in Camellia oleifera'Xianglin XLC15'were examined by microscope.The results are showed that the pollen germination occurred in 2 h after pollination,the pollen tubes grew up to the base of the style in 48 h after pollination.And then the pollen tubes entered into the embryo sac through a degenerated synergid,which belonged to the porogamy type.About 23% of the pollen tubes were observed to penetrate into the embryo sac.The fusion of sperm cells and polar nucleus mainly occurr...