National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31570447, 31170426]; Specialized Research Fund for the Doctoral Program of Higher EducationSpecialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [20124321110006]; Program for Science and Technology Innovative Research Team in Higher Educational Institutions in Hunan Province
机构署名：
本校为第一且通讯机构
院系归属：
生命科学与技术学院
摘要：
Background and aims: Direct measurement of fine root diversity is very important to unveil belowground interaction, community dynamics and ecosystem functions in forests, but is limited by the effective method. This study attempted to develop specific primers for a quantitative real-time PCR (qPCR) method to determine diversity of fine roots and test whether this method could be applied in field forests. Methods: We used inter-simple sequence repeat (ISSR) analyses to develop the specific primers and applied the qPCR method to determine the bel...
