期刊论文

Interaction Between Epigallocatechin Gallate and Rice Bran Protein

Wu, W.

中文

中国粮油学报

1003-0174

2019

34

12

19-26

10.3969/j.issn.1003-0174.2019.12.005

国家自然科学基金面上项目（31771918）；

本校为第一且通讯机构

采用荧光光谱和紫外-可见光谱法研究表没食子儿茶素没食子酸酯(Epigallocatechin gallate,EGCG)和米糠蛋白(Rice bran protein,RBP)之间的相互作用.结果表明:EGCG对RBP有较强的荧光猝灭作用,为且动态和静态混合淬灭.EGCG与RBP在290、300、310 K时相互作用的表观结合常数分别为1.322 8× 106、1.504 2×106、1.817 6×106 L/mol,对应的结合位点数分别为1.387 8、1.327 0、1.377 0.同步荧光光谱、紫外-可见光谱和三维荧光光谱表明EGCG主要影响RBP色氨酸残基空间结构的微环境,使其周围的疏水性下降.热力学参数表明EGCG与RBP结合的主要作用力为疏水相互作用.根据F(o)ster非辐射转移理论计算了不同条...

The interaction between epigallocatechin gallate (EGCG) and rice bran protein(RBP)was investigated by fluorescence and ultraviolet-visible spectroscopy. The results indicated that EGCG had a strong ability to quench the fluorescence of RBP in the mixed of dynamic mode and static mode. The binding constants (KA) and site numbers (n) obtained at different temperatures were 1.322 8×106L/moL, 1.387 8 (290 K); 1.504 2×106 L/moL, 1.3270 (300 K); 1.817 6×106 L/moL, 1.377 0 (310 K), respectively. Synchronous fluorescence spectroscopy, ultraviolet-vi...