期刊论文

Deng, Kequan

中文

中国粮油学报

1003-0174

2015

30

5

19-23,28

10.3969/j.issn.1003-0174.2015.05.004

31201319:国家自然科学基金 201303071:公益性行业(农业)科研专项

本校为第一机构

以干热预处理低温脱脂豆粕和溶剂浸提预处理低温脱脂豆粕为原料制备大豆球蛋白,研究脱脂豆粕预处理对制备大豆球蛋白结构的影响。结果表明,相比对照低温脱脂豆粕,干热预处理使得大豆球蛋白氧化程度增加,而溶剂浸提预处理则减小大豆球蛋白的氧化程度。随着蛋白质氧化程度的增加,大豆球蛋白游离巯基和总巯基含量、二级结构中α-螺旋和β-折叠含量、表面疏水性以及内源荧光强度下降,内源荧光最大吸收峰发生蓝移,并且伴随着氧化聚集体的出现。

Low-temperature defatted soybean meal treated by dry heating preprocessing and solvent extraction were used as material to prepare soybean glycinin. Effects of pretreatment of defatted soybean meal on structure of soybean glycinin were investigated. The results showed that compared to low-temperature defatted soybean meal, defatted soybean meal produced by dry heating preprocessing increased oxidation extent of soybean glycinin, and solvent extraction of defatted soybean meal reduced oxidation extent of soybean glycinin. The increased oxidation of soybean glycinin resulted in loss of free sulp...