期刊论文

中文

中国粮油学报

1003-0174

2010

25

7

80-84,89

中南林业科技大学青年基金资助项目（101-0594）；

本校为第一机构

以油茶粕蛋白为原料, 研究了其最适水解酶, 并对酶解工艺进行了优化. 研究结果表明, 油茶粕蛋白酶解的最适酶为2709碱性蛋白酶. 以多肽得率为指标, 运用Design-Expert7.1Trial统计分析软件, 设计了4因素3水平试验, 得到了油茶粕蛋白酶解的最优条件, pH值为8.55, 酶用量为66.38 mg/g, 温度为 45.80℃, 水解时间为5.24 h, 在此条件下, 预测多肽得率为39.72％

To hydrolyze the protein of oil - tea - seed meal by protease,the optimum enzyme and reaction conditions were determined and optimized.Using polypeptides yield as evaluation index,a four factors and three levels＇test was designed to optimum the conditions of enzymatic hydrolysis of oil - tea - seed meal protein with response surface methodology.Results：2709 alkaline protease is the best enzyme.The obtained optimum process parameters are pH 8. 55,enzyme dosage 66.38 mg/g,temperature 45.80℃,and time 5.24 h...