期刊论文

中文

理化检验-化学分册

1001-4020

2009

45

10

1191-1193

广西大学科研基金资助项目（CC040020）；

本校为其他机构

采用福林(Folin-Ciocalteu)试剂光度法测定马占相思树叶提取液中多酚的含量.试样用乙醇(体积分数为60%)提取.分取部分提取液在加入福林试剂与100 g·L-1碳酸钠溶液(1+9)的混合液20 mL,反应时间2 h,温度30℃的条件下进行显色,并于760 nm波长处测定其吸光度,以儿茶素为标样,多酚质量浓度在0.8～9.6 mg·L-1范围内呈线性关系.在同一样品的萃取液中分取9份试液,分别加入儿茶素标准液,按方法操作求得方法的回收率为98.3%～102.9%.从同一试样萃取液中分取5份进行测定,求得其相对标准偏差为1.47%.

Leaves of acacica mangium was powdered and extracted with ethanol （φ60%）. An aliquot of the ethanol extract was reacted with a mixturecontaining 2 mL of the Folin-Ciocalteu reagent and 18 mL of 100 g · L^-1 Na2 CO3 solution for 2 h at 30 ℃. The absorbance of the colored solution was measured at the wavelength of 760 nm with 1 cm absorption cell against reagent blank as reference. Catachin standard solution （100 mg · L^-1 in absolute alcohol） was used as standard for polyphenol in preparation of standard curve. Range of linearity of the ...