Selection of potential reference genes for RT-qPCR in the plant pathogenic fungus Colletotrichum fructicola

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成果类型：

期刊论文

作者：

Chen, Xingzhou;Chen, Xinggang;Tan, Qian;He, Yuan;Wang, Zhikai;...

通讯作者：

Liu, J.;Zhou, G.

作者机构：

[Liu, Junang; He, Yuan; Zhou, Guoying; Wang, Zhikai; Tan, Qian; Chen, Xinggang; Chen, Xingzhou] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Peoples R China.

[Liu, Junang; He, Yuan; Zhou, Guoying; Wang, Zhikai; Tan, Qian; Chen, Xinggang; Chen, Xingzhou] Cent South Univ Forestry & Technol, Key Lab Natl Forestry & Grassland Adm Control Arti, Changsha, Peoples R China.

[Liu, Junang; He, Yuan; Zhou, Guoying; Wang, Zhikai; Tan, Qian; Chen, Xinggang; Chen, Xingzhou] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha, Peoples R China.

通讯机构：

[Zhou, G.; Liu, J.] K

Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, China

语种：

英文

关键词：

Colletotrichum fructicola;RT-qPCR;Camellia oleifera;reference gene;Transcriptome

期刊：

Frontiers in Microbiology

ISSN：

1664-302X

年：

2022

卷：

页码：

982748

DOI：

10.3389/fmicb.2022.982748

基金类别：

This research was funded by the National Natural Science Foundation of China (31971661); Natural Science Foundation of Hunan Province (2021JJ31145); Forestry Science and Technology Innovation Fund Project of Hunan Province (XLK202101-2); and Camellia oleifera Industrial Research Demonstration Project of Hunan Province (XiangCaiZiHuanZhi [2021] No. 9).

机构署名：

本校为第一机构

院系归属：

林学院

摘要：

Colletotrichum is widespread, and these pathogenic fungi can cause various plant diseases. Studies have shown that Colletotrichum fructicola cause oil-tea (Camellia oleifera) anthracnose and is widely distributed as a dominant fungus in all Ca. oleifera-producing regions. Real-time quantitative PCR(RT-qPCR) is considered the most reliable technique for simultaneously measuring relative gene expression levels in different tissues. Target genes are typically quantified using RT-qPCR to explore gene function, and reliable RT-qPCR results require data normalization using stable reference genes. No...

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Selection of potential reference genes for RT-qPCR in the plant pathogenic fungus Colletotrichum fructicola

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