2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010)
ISSN：
2151-7614
年：
2015
页码：
530-533
会议名称：
International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2010)
会议论文集名称：
2010 4th International Conference on Bioinformatics and Biomedical Engineering. [v.1.2]
会议时间：
201
会议地点：
Chengdu, China
会议主办单位：
[Zhang, Dang-Quan;Gu, Zhen-Jun;Deng, Shun-Yang;Fan, Shao-Gang;Zhu, Quan-Dong] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod State Forestry Adm, Biotechnol Core Facil, Changsha 410004, Hunan, Peoples R China.
会议赞助商：
IEEE Engn Med & Biol Soc, Sichuan Univ, Wuhan Univ
出版地：
345 E 47TH ST, NEW YORK, NY 10017 USA
出版者：
IEEE
ISBN：
978-1-4244-4713-8
基金类别：
Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30972343, 30700643]; Hunan Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [05JJ40125]
机构署名：
本校为第一且通讯机构
院系归属：
林学院
生命科学与技术学院
摘要：
The transformation of cDNA into plant is still not carried out directly by a universal transgenic vector, hence it is not convenient for the transgenic application of those function-important cDNAs. Herein we introduced a rapid, cost-effective PCR-ba sed DNA synthesis method to construct a new universal plant overexpression vector for the direct inserting of target cDNA during transgenic application. The new engineered transgenic vector was based on the pCAMBIA vector backbone, which was inserted by five motifs, including a promoter, an enhancer, a Linker DNA fragment, a endoplasmic reticulum ...
