会议论文

Establishment of Genetic Transformation System and Over-expression of PmgFT Gene in Rosa rugosa

中文

2018

209-216

中国园艺学会观赏园艺专业委员会2014年学术年会

中国园艺学会观赏园艺专业委员会2014年学术年会论文集

20140716

青岛

中国园艺学会观赏园艺专业委员会

本校为第一机构

本实验分别以玫瑰的未展开小叶和体细胞胚为转化受体材料,以GUS基因作为报告基因,对玫瑰的转化体系进行了研究.结果表明,以未展开小叶诱导不定芽再生作为转化受体系统并不适用于玫瑰的遗传转化,无法获得转基因的植株;而以次生胚增殖萌发再生为转化受体系统进行遗传转化的效果较好,可以获得转基因植株,并且通过不同大小的体细胞胚转化效果的比较发现,0.02～0.04g的体细胞胚团作为转化受体的转化效率最高,这些转基因株系通过了GUS的染色检测和PCR、southern杂交分子检测,证明了以体细胞胚为转化受体材料的可行性,也表明玫瑰遗传转化体系的成功建立.以此转化体系为基础,又将梅花的FT基因(PmgFT)转入...

　　The comparison of genetic transformation via directly organogenesis and secondary somatic embryogenesis was made in this research.The results indicated that transgenic plantlets cannot be obtained by genetic transformation via directly organogenesis.On the contrary, transgenic plantlets were successfully developed by Agrobacterium-mediated transformation via secondary somatic embryogenesis.In this transformation process, 0.02-0.04 g tufted somatic embryos were proved to be the optimal types for transformation.The transgenic plantlets obtain...