Chemical speciation of heavy metal is hot topic in soil science, plant nutrition, and environmental science. Many scientists have developed many different sequential extraction procedures for chemical fractions. Assessment of bioavailability of heavy metal in contaminated soils with chemical fractionation, it is significance for us to fully understand the harms of heavy metals, and treat heavy metal contaminated soils. The result showed that the water soluble fraction and exchangeable species of metals are quite available for plants. However, the metals with residual fraction are the most diff...