会议论文

Study on isolation screening and identification of endogenous microorganisms to control Poplar canker

中文

2016

136-148

第三届中国林业学术大会

第三届中国林业学术大会论文集

201309

福州

中国林学会<&wdkj&>国家林业局<&wdkj&>福建省人民政府

本校为第一机构

本研究旨在从杨树不同部位组织中分离出对杨树溃疡病有良好生防效果的拮抗微生物.通过平板对峙初筛,发酵液“三明治法”和“改良琼脂扩散法”复筛以及离体组织防治效果测定,从115株杨树内生菌中筛选出一株杨树溃疡病拮抗真菌,编号为YGF9,其对杨树离体组织溃疡病防治效果可达76.47％;经形态学观察和ITS序列及其系统发育树分析,鉴定该菌株为黄绿木霉(Trichoderma aureoviride).该菌株的发现为杨树溃疡病的生物防治提供了新的原材料,为后续生物源农药的开发利用奠定了基础.

　　The aim of this study is to find and isolate antagonistic microorganisms which have good bio-control effect to poplar canker from the different parts of the poplar organizations.The strain YGF9 was selected from 115 strains of poplar endogenous microorganisms.The strain YGF9 showed good antagonistic activity in plate confrontation initial screening and fermentation medium "sandwich method" and "improved agar diffusion method " multiple screening.Through the in vitro tissue experiment,the control effect of strain YGF9 to poplar canker could ...