In this paper,the optimum fermenting conditions of fermentation were studied using grass carp and rice powder as main materials and using Lactobacillus as fermenting starter culture.Using the content of total acid as evaluation index,a four factors and three levels' test was designed.The optimum condition of fermentation was studied with response surface methodology.The obtained optimum fermenting parameter was rice powder 42.45%,starter culture dosage 2.09×106 cfu/g,temperature 30.56 ℃,time 18.47 d,the prodicted content of total acid was 15...