Based on molecular imprinting and nanotechnology, a highly specific and sensitive electrochemical aptasensor was proposed for assaying kanamycin (KAN). First, chitosan-graphene (CG) composite multilayer films and Au nanoparticles (AuNPs) were applied to modify a glassy carbon electrode (GCE) for augmented “electron antennae». Then, we constructed a KAN sensor using functional monomer of 3-aminophenylboronic acid (3-APBA) as trap. Au@Fe3O4 nanocomposites, Fe3O4 nanoparticles (Fe3O4NPs) loaded with AuNPs, were dual functionally modified with β...