The invention discloses a wood-UHPC (Ultra High Performance Concrete) combined box girder. The wood-UHPC combined box girder comprises a UHPC top plate, a laminated wood web, a laminated wood bottom plate, a laminated wood cross beam arranged at the fulcrum position of a girder body, wherein the laminated wood bottom plate, the laminated wood cross beam and the laminated wood web are connected through transverse prestressed reinforcement, and the laminated wood web is connected with the UHPC top plate through a shearing force connecting part. The invention further relates to a preparation meth...