期刊论文

Cloning of a CAC Gene in Dove Tree(Davidia involucrata Baill.)and Evaluating its Potential as a Novel Reference Gene

中文

生物技术通报

1002-5464

2017

33

4

119-129

10.13560/j.cnki.biotech.bull.1985.2017.04.015

(0490016):中南林业科技大学博士后科研基金项目 (QJ201512):中南林业科技大学青年基金项目

本校为第一机构

从珙桐(Davidia involucrata Baill.)中克隆到一个编码网格蛋白衔接蛋白复合物(Clathrin adaptor complexes,CAC)的基因,命名为DiCAC(GenBank登录号为KX268525)。该基因开放阅读框全长1 317 bp,编码438个氨基酸。同源序列比对与聚类分析表明,该基因与其它15种植物的CAC氨基酸序列的相似度达到94%以上,其中与葡萄CAC的氨基酸序列同源性最高。为评价该基因作为珙桐内参基因的可行性,结合5个珙桐管家基因(ACT7、EF1a、GAPDH、β-TUB、18S rRNA),采用半定量PCR和实时荧光定量PCR对DiCAC基因在珙桐不同组织以及不同发育阶段苞片中的表达稳定性进行分析,并与常见管家基因的稳定性进行比较,结果表明,该...

In this research,a gene named as DiCAC(GenBank accession number KX268525),encoding the clathrin adaptor complexes(CAC),has been cloned from dove tree(Davidia involucrata Baill.). The sequence of DiCAC includes a ORF(open reading frame)of 1317 bp,which encodes a protein with 438 amino acids. Homologous alignment and cluster analysis show that DiCAC shared over 94% amino acid sequence identity with CAC genes in other 15 plants,and it has the highest amino acid homology with grape(Vitis vinifera)CAC gene. To evaluate the feasibility of using DiCAC...