期刊论文

Changes of Endogenous Hormones During the Culture of Callus from Sopatholobus suberechtus

中文

生物技术通报

1002-5464

2017

33

3

66-70

10.13560/j.cnki.biotech.bull.1985.2017.03.010

国家林业公益性行业科技专项（201304807）；

本校为第一机构

旨在建立密花豆组织培养再生体系并测定其再生过程中内源激素的动态变化。利用酶联免疫吸附法(ELISA)对密花豆嫩叶愈伤组织诱导与不定芽分化过程中的4种内源激素:生长素(IAA)、脱落酸(ABA)、玉米素(ZT)和赤霉素(GA_3)的含量进行测定和分析。结果表明,在愈伤组织的诱导过程中,IAA的含量始终处于较高水平,并在愈伤组织生长最旺时达最大,而ABA、ZT和GA_3的含量一直保持在较低水平;IAA/ABA对愈伤组织的诱导与生长有着重要的调节作用。在愈伤组织的分化过程中,IAA的含量呈先降后升,并在分化成芽期达到最高,且高于愈伤组织诱导期,介于1.15-1.87 μg/g之间;ZT和ABA的含量均呈先升后降趋势,而GA_3则一直...

This work is to establish a regenerative system of Sopatholobus suberechtus tissue and determine the dynamic changes of endogenous hormones during the regeneration process. The enzyme-linked immunosorbent assay （ ELISA ） was used to determine and analyze the dynamic changes of four endogenous hormones （ IAA, ABA, ZT, and GA3 ） contents during the callus induction and differentiation. As results, the content of IAA always kept at a high level and reached maximum when the callus growth was the most exuberant in the process of callus induction...