会议论文

Cloning and Sequence Analysis of a Fatty Acid Hydroperoxide Lyase Gene from Camellia oleifera

中文

2012

1-9

中国林学会经济林分会2012年学术年会

中国林学会经济林分会2012年学术年会论文集

2012-11-01

浙江临安

中国林学会

本校为第一机构

　　以油茶近成熟种子为材料,根据油茶EST 文库中已知的脂氢过氧化物裂解酶EST 序列,利用3’RACE 与5’RACE 技术,克隆到脂氢过氧化物裂解酶的全长cDNA序列.该基因全长1648bp,,包含一个1476bp 开放阅读框长,编码491个氨基酸,5’与3’非编码分别为52bp、121bp.预测该蛋白相对分子量为54.7779KDa,等电点为6.77,是个叶绿体转运肽,N 端包含有22个疏水氨基酸残基组成的序列,不仅具有转运肽富含的丝氨酸,还含有大量转运肽中很少存在的脯氨酸.多序列比对发现油茶脂氢过氧化物裂解酶核苷酸序列与茶的相似性达到96％,因此将该基因命名co HPL.

　　A cDNA library and a of Camellia oleifera were constructed.According to the fatty acid hydroperoxide lyase EST sequences of Camellia oleifera,a specific primer was designed,the fatty acid hydroperoxide lyase gene was cloned from camellia oleifera with 3’RACE and 5’RACE,respectively.This gene is 1648bp in length,including 51bp of 5’untranslated region and 121bp of 3’untranslated region.It has an open reading frame of 1476bp,encoding aprotein of 491 amino acid residues with protein molecular weight of 54777.9Da and isoelectric point of 6....