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Green-emissive carbon dots for multitarget staining of cells, fungi, and bacteria: Insights into mechanisms and clinical applications

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成果类型:
期刊论文
作者:
Sun, Jingbo;Chen, Liushunhao;Li, Hongchen;Xu, Chen;Xu, Dong*;...
通讯作者:
Xu, Dong
作者机构:
[Li, Hongchen; Xu, Chen; Ren, Jiali; Xu, Dong; Sun, Jingbo; Chen, Liushunhao] National Engineering Research Centre for Deep Processing of Rice and By-products, Central South University of Forestry & Technology, Changsha, 410004, China
[Xu, Dong] The Faculty of Food Science and Engineering, Central South University of Forestry & Technology, Changsha, 410004, China. Electronic address: philip198349@gmail.com
[Ren, Jiali] The Faculty of Food Science and Engineering, Central South University of Forestry & Technology, Changsha, 410004, China
通讯机构:
[Dong Xu] N
National Engineering Research Centre for Deep Processing of Rice and By-products, Central South University of Forestry & Technology, Changsha, 410004, China<&wdkj&>The Faculty of Food Science and Engineering, Central South University of Forestry & Technology, Changsha, 410004, China
语种:
英文
关键词:
Carbon dot;Fluorescence enhancement;Microorganism;Nucleus;Staining
期刊:
Talanta
ISSN:
0039-9140
年:
2026
卷:
297
期:
Pt A
页码:
128625
基金类别:
CRediT authorship contribution statement Jingbo Sun: Writing – original draft, Methodology, Investigation. Liushunhao Chen: Writing – review & editing, Project administration, Investigation, acquisition. Hongchen Li: Methodology, Investigation. Chen Xu: Methodology, Investigation. Dong Xu: Writing – review & editing, Supervision, Project administration, acquisition, Data curation, Conceptualization. Jiali Ren: Writing – review & editing, Supervision, Project administration, Investigation, acquisition. Acknowledgements This work was supported by funding from the Open Fund of the State Key Laboratory of Chemo/Biosensing and Chemometrics ( Hunan University , 2021004 ), the National Natural Science Foundation of China ( 21505162 ), the Grain-Oil Process and Quality Control 2011 Collaborative and Innovative Grant from Hunan Province, Natural Science Foundation of Hunan Province , China ( 2020RC3042 , 2022SK2102 ), and Hunan Postgraduate Science and Technology Innovation Fund Project ( CX202102068 ).
机构署名:
本校为第一且通讯机构
摘要:
Biological staining is essential for imaging, functional investigation, and disease diagnostics. In this research, innovative green-emissive carbon dots (G-CDs) were hydrothermally prepared from 1,2,4-triaminobenzene and ascorbic acid (AA) and further explored for versatile imaging of mammalian cells, plant cells, and microorganisms. The G-CDs, exhibiting fluorescence enhancement, outperform conventional dyes in terms of staining efficiency and specificity across various biological systems. For mammalian cells (SH-SY5Y, Hep3B, and HEK293), the G-CDs can stain both the nuclei and nucleoli in 10...

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