Background: On-site and rapid detection of glucose level in foods is important for monitoring and control glucose intake. Methods: Magnetic and N-doped nanofibrous carbon microspheres (Fe@NCMs) with hierarchical porosity were designed as a novel nanozyme (Fe@NCMzyme), and the catalytic mechanism was investigated via the density functional theory (DFT) calculation. Therefore, Fe@NCMzyme sensor and point-of-care testing (POCT) platform were developed for glucose detection in food samples. Significant findings: Fe@NCMzyme showed 3D porous structure, a larger surface area, and N-doped carbon mater...