期刊论文、会议论文

中文

种子

1001-4705

2010

29

1

24-27

第二届中国林业学术大会——S9 木本粮油产业化

2009-11-07

中国广西南宁

10.16590/j.cnki.1001-4705.2010.01.085

湖南省教育厅科学研究项目(08C931)

本校为第一机构

采用ISSR分子标记技术对5个按树原种和3个推广的杂交桉树品种进行基因组DNA多态性分析.从100条引物中筛选出10条用于ISSR-PCR扩增,共扩增出155务DNA条带,其中多态性条带148条,占总条带数的95.5%.通过NTSYSpc2.10e软件分析ISSR扩增结果,结果表明,桉树资源具有较丰富的遗传多样性,8份桉树材料的遗传距离(GD)值范围为0.4195～1.003 4,遗传相似性(SM)值范围为0.4200～0.693 3.应用UPGMA法进行聚类分析,将8份桉树材料划分为3个大类,邓恩桉和赤桉为一类;巨尾桉、尾巨桉、尾细桉、韦塔桉、粗皮按为一类;托里桉则独成一类.

3 Eucalyptus original breeds and 5 Eucalyptus crossbreeds were used as materials to analyze genome polymorphism with ISSR markers. 10 primers screened out from 100 primers were used for ISSR-PCR. A total of 155 bands were generated, of which 148 bands were polymorphic bands （ the percentage of polymorphic band, PPB = 95.5% ）. The results of ISSR were analyzed by NTSYSpc 2. 10e software, and indicated that there actually existed much genetic diversity at molecular level among the Eucalyptus species. Genetic distance （GD） of 8 Eucalyptus mate...