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Differentially Expressed cDNAs in Cold-stressed SSH Library of Ceratoides lanata

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成果类型:
期刊论文、会议论文
作者:
Zeng, Yan-Ling;Zhang, Dang-Quan*;Ming, Fu-Huan;Song, Zhi-Dan;Han, Xin;...
通讯作者:
Zhang, Dang-Quan
作者机构:
[Zhang, Dang-Quan; Han, Xin; Song, Zhi-Dan; Ming, Fu-Huan; Zeng, Yan-Ling] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod State Forestry Adm, Biotechnol Core Facil, Changsha 410004, Hunan, Peoples R China.
[Jiang, Ping] Lanzhou Univ, Sch Civil Engn & Mech, Lanzhou 730000, Peoples R China.
通讯机构:
[Zhang, Dang-Quan] C
Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod State Forestry Adm, Biotechnol Core Facil, Changsha 410004, Hunan, Peoples R China.
语种:
英文
关键词:
Ceratoides lanata;SSH library;Cold stress;Antifreeze gene;cDNA cloning;EST;genetic engineering
期刊:
2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010)
ISSN:
2151-7614
年:
2010
页码:
1-3
会议名称:
4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE)
会议论文集名称:
International Conference on Bioinformatics and Biomedical Engineering
会议时间:
JUN 18-20, 2010
会议地点:
Chengdu, PEOPLES R CHINA
会议主办单位:
[Zeng, Yan-Ling;Zhang, Dang-Quan;Ming, Fu-Huan;Song, Zhi-Dan;Han, Xin] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod State Forestry Adm, Biotechnol Core Facil, Changsha 410004, Hunan, Peoples R China.^[Jiang, Ping] Lanzhou Univ, Sch Civil Engn & Mech, Lanzhou 730000, Peoples R China.
会议赞助商:
IEEE Engn Med & Biol Soc, Sichuan Univ, Wuhan Univ
出版地:
345 E 47TH ST, NEW YORK, NY 10017 USA
出版者:
IEEE
ISBN:
978-1-4244-4713-8
基金类别:
Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30700643, 30972343]; Hunan Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [05JJ40125]
机构署名:
本校为第一且通讯机构
院系归属:
林学院
生命科学与技术学院
摘要:
As a high stress resistance psammophyte with the characteristic of sand fixation and winterfat, Ceratoides lanata had been successfully introduced into many countries in recent years. The leaves and twigs of Ceratoides lanata in room temperature were used as the Driver and those treated by cold stress were used as Tester. Tester and Driver ds cDNA were prepared from their high quality mRNA which were purified from their total RNA. Tester and Driver cDNA were separately digested to obtain shorter, blunt-ended molecules by RasI. Then two Tester populations are created with different adaptors, wh...

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