期刊论文

Study on adsorption properties and mechanism of As~(3+) by an arsenic-resistant Bacillus strain

中文

环境科学学报

0253-2468

2020

40

8

2758-2770

10.13671/j.hjkxxb.2020.0121

No.2016YFD0800805-4:国家重点研发计划 No.21707169:国家自然科学基金 No.2017SK2273:湖南省重点研发计划项目 2019SK2191:湖南省重点研发计划项目 No.19K107:湖南省高等学校创新平台开放基金 No.19C1900:湖南省教育厅科学研究项目

本校为第一机构

为了给砷污染水体的微生物修复提供理想材料和必要的理论依据,从湖南某矿区筛选分离得到一株高耐砷菌株,利用16S rDNA基因测序分析对其进行鉴定.同时,采用单因素试验研究了耐性菌吸附As~(3+)的影响因素及规律,通过研究等温吸附属性、吸附动力学和热力学属性,分析亚细胞赋存特性,并结合扫描电镜(SEM)、傅里叶红外光谱(FTIR)等技术手段,初步探讨了吸附发生的可能机理.结果表明,通过形态学、生理生化及分子鉴定,初步鉴定为芽孢杆菌属(Bacillus sp.),命名为Bacillus sp.strain AsT4.该菌株固体平板培养对砷的耐受阈值为40mmol·L~(-1);菌株最适生长条件为温度35℃、pH=7.0、NaCl浓度5g·L~(-1)、转速...

In order to provide ideal materials and necessary theoretical basis for microbial remediation of arsenic pullution, a high As-resistance strain was isolated from a mining area in Hunan province, and was identified by 16S rDNA gene sequencing analysis. The influencing factors of As3+ adsorption by bacterial were studied by single factor experiment. The possible mechanism of adsorption was preliminarily explored by studying adsorption isotherm, kinetics and thermodynamics; the studying of subcellular distribution; and the observation with scannin...