期刊论文

Isolation and characterization of a cadmium-resistant bacterial strain ( Burkholderia cepacia) and its cadmium-absorption and transport properties

Zhu, J.

中文

环境科学学报

0253-2468

2020

40

6

2195-2204

10.13671/j.hjkxxb.2020.0054

No.2016YFD0800805-4:国家重点研发计划 No.21707169:国家自然科学基金 No.2019SK2191:湖南省重点研发计划 2017SK2273:湖南省重点研发计划 2016SK2030:湖南省重点研发计划 No.19K107:湖南省高等学校创新平台开放基金 No.19C1900:湖南省教育厅科学研究项目

本校为第一且通讯机构

农田土壤镉( Cd)污染日益加剧,严重威胁着农村生态安全与居民身体健康,而微生物修复被认为是一种行之有效的Cd污染土壤原位修复方法.为了给Cd污染土壤微生物修复提供功能菌株,采取平板划线从Cd污染土壤中分离纯化得到一株高耐性耐Cd菌株B-6,通过生理生化试验及16S rDNA对其进行了鉴定,并研究了菌株对Cd~(2+)的吸收转运特性.经鉴定,高耐性耐Cd菌株B-6为伯克霍尔德菌属( Burkholderia cepacia),其对Cd~(2+)的耐受阈值为850 mg·L~(-1).通过单因素优化培养发现,菌株B-6对Cd~(2+)的吸收量随着培养温度的增加先增大后减小;随着培养液初始pH值的增大先增大后减小,同时发现菌株B-6能够有效调控系统的终...

Cadmium pollution in farmland soil is increasing and seriously threatens rural ecological security and residents' health. Microbial remediation is considered to be an effective method for in situ remediation of the cadmium contaminated soils. In order for bioremediation of the cadmium-contaminated soil, a highly cadmium-resistant bacterial strain was isolated and purified from the cadmium-contaminated soil by physiological, biochemical tests and 16S rDNA analysis. The cadmium-absorption and transport properties of the bacterial strain were char...