期刊论文

中文

环境科学

0250-3301

2015

36

10

3839-3844

10.13227/j.hjkx.2015.10.037

12K071:湖南省教育厅重点实验室开放基金 41301275:国家自然科学基金 41430860:国家自然科学基金 :湖湘青年科技创新创业平台培养对象自选项目

本校为第一机构

土壤微生物在推动土壤碳循环过程方面发挥着重要作用,然而种植方式的改变对土壤微生物多样性的影响机制还不十分清楚. 本研究采集湖南省盘塘县长期定位试验站红壤稻田(PR)、 旱地(UC)及水旱轮作(PR)这3种不同种植方式的土壤样品,采用末端限制性酶切片段长度多态性分析(T-RFLP)技术和实时荧光定量(RT-PCR)技术分析了土壤细菌16S rRNA基因的多样性和丰度,研究种植方式改变对土壤微生物数量、 群落结构及其多样性的影响. 结果表明, 3种种植方式的土壤细菌16S rRNA基因数量(以干土计)为2.5×10~9~1.5×10~(10)拷贝·g~(-1),与PR相比,UP和UC处理16S rRNA基因丰度显著下降(P<0.05). 同时,3种种植方...

Soil microbe plays an important role in carbon cycling, however, the effect of land use on soil microbe remain unclear. In present study, soil samples were collected from a long-term field experiment (Pantang Agroecosystem) in subtropical China (established in 1989), including paddy-rice (PR), upland-crop (UC), and paddy rice-upland crop rotation (PU) on soil bacterial (bacteria and Archaea) community structures. The effects of long-term different land uses were determined using terminal restriction fragment length polymorphism (T-RFLP) and quantitative PCR (RT-PCR) of the 16S rRNA gene. The a...