期刊论文

中文

环境科学

0250-3301

2011

32

8

2397-2402

10.13227/j.hjkx.2011.08.023

200909066:环保公益性行业科研专项 2009ZX07212-001-2:水体污染控制与治理科技重大专项 09K055:湖南省高等学校创新平台开放基金 2010SK3046:湖南省科技厅科技项目 2006180:湖南省环境科学重点学科建设项目

本校为第一机构

采用选择性平板培养基法和PCR-DGGE法,比较研究了人工湿地污水处理系统中植物套种模式和无植物单元在不同季节和不同单元之间根际微生物多样性.结果表明,植物套种模式的根际微生物数量明显高于无植物模式,夏季明显多于冬季.在三级串联的处理单元之间,夏季根际微生物数量呈现＂少-多-少＂的变化规律,冬季无明显的变化规律.UPGMA聚类分析显示,除冬季个别样外,相同季节的处理均聚集在同一个分支下,且微生物多样性指数夏季高于冬季.得到的5个差异序列（DF1～DF5）,经BLAST比较,分别找到源性最近的已知类群,分别为大肠杆菌、柠檬酸细菌、变形菌、产酸克雷伯菌、伯克霍尔德氏菌.BLASTX比较发现DF1与分...

The selective culture method and PCR-DGGE technology were applied to analyze the number and the biodiversity of micro-organism species in cells with plant intercropping models and without plants in different seasons in a wetland system constructed for treatment of municipal sewage.The results showed that the numbers of microorganisms were considerably larger in the cells with plant intercropping models than those without plants,while the number of microorganisms was apparently larger in summer than that in winter in all treatments.Along the thr...