期刊论文

IDENTIFICATION AND ANALYSIS OF 14 SAMPLES IN PRUNUS SIMONII BY ISSR MOLECULAR MARKER

中文

内蒙古农业大学学报(自然科学版)

1009-3575

2010

31

2

147-153

国家公益性行业科研专项(200904049)； 湖南省教育厅项目； 湖南省科技计划项目；

本校为第一机构

采用ISSR分子标记,对14个杏李品种进行了遗传多样性分析,从100条ISSR引物中筛选出多态性较高的22条引物对14个杏李品种进行扩增,共扩增出205个位点,多态性位点比率为81.95%;建立了11个内眼无法区分的杏李品种的分子识别卡,用3条引物的3个特异性识别位点即可将11个杏李品种区分开;聚类分析结果显示:14个杏李品种基因型间的平均遗传距离(GD)为4.6632,味馨与其它品种之间平均遗传距离最大,为10.332,风味玫瑰与味厚之间遗传距离最小,为2.3462;UPGMA聚类分析可将14个供试品种分为两组,即含有杏基因75%李基因25%的‘味馨'单独分为1组,其他品种均含李基因75%以上杏基因25%以下,聚为另1组;第2类进一步可...

Genetic variation analysis of 14 samples in Prunus Simonii have been studied by adopting ISSR markers method.22 primers out of 100 have been screened out and 205 polymorphic locis were amplified,The polymorphic loci percentage was 81.95%;Molecular identification card was build to distinguish the 11samples in Prunus Simonii that can not distinguish by eyes,three polymorphic locis of three primers could detach the 11 cultivars;Clustering analysis showed the average genetic distance between genotypes of the 14 samples is 4.6632,＇Weixin＇has the l...