期刊论文

LDENTIFICATION OF 13 SAMPLES IN PYRUS PYRIFOLIA BY ISSR MOLECULAR MARKER

中文

内蒙古农业大学学报(自然科学版)

1009-3575

2009

30

4

29-35

国家公益性行业科研专项（200904049）； 湖南省教育厅项目； 湖南省科技计划项目；

本校为第一机构

用ISSR分子标记对13个砂梨品种进行了遗传多样性分析,从50条ISSR引物中筛选出多态性好、稳定性高的12条引物对13个梨品种的DNA序列进行了PCR扩增,共扩增出92个位点,多态性位点比率为63.04%;建立了13个砂梨品种的分子识别卡,用2条引物的9个多态性位点即可将13砂梨品种分开来;采用UPGMA聚类方法构建了各品种之间的聚类图,结果显示,新世纪梨作亲本的品种与非新世界梨做亲本的品种间可明显划分为2组,可进一步细分为6个亚组。本研究为砂梨品种之间的杂交实验和梨新品种培育以及新世纪梨为原料加工产品的真伪鉴别提供了可靠的理论依据。

Genetic differences and kindredship among 13 samples in pyrus pyrifolia have been studied by adopting ISSR markers method.12 out of 50 ISSR primers have been screened out and 58 ISSR markers were amplified from these clones with 92 polymorphic loci by PCR,proportion of polymorphic loci is 63.04%.Identification card of 13 pyrus pyrifolia cultivars was built,nine polymorphic locis of two primers could distinguish the 13 pyrus pyrifolia cultivars.PCR amplification showed that they could be classified into 2 groups and 6 subgroups by UPGMA phylogenetic analysis.The average genetic distance between...