Beer brewers spent barley grain was pretreated by wet grinding and dilute H_2SO_4 prehydrolysisbefore enzymatic hydrolysis of the residual fibers. About 50%of the proteins could be removed from spentgrain in wet grinding,and sugar yleld of enzymatic hydrolysis could also be increased by l4%. In the acidprehydrolysis with H_2SO_40.75%and ratio of solid to liquid l:8～1:10,the optimal reaction tirnes were80～100 min at 121～125℃ or 15～20 min at 150℃. 95%sugar yields from starch and pentose in the pre-hydrolysis,76.4%from cellulose in the enzym...
