期刊论文

Zhou, Q.

中文

植物资源与环境学报

1674-7895

2009

18

1

74-79

10.3969/j.issn.1674-7895.2009.01.014

国家自然科学基金资助项目（30570226） 湖南省自然科学基金重点项目（06JJ20099） 湖南省教育厅基金项目（05C336）

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采用硅胶柱层析法对苍耳(Xanthium sibiricum Patrin.)叶甲醇提取物的乙酸乙酯萃取物的活性成分进行了跟踪分离及生物活性测定.共分离出8个活性组分,其中组分Ⅱ对试虫的生物活性最高.组分Ⅱ(0.01 g·mL~(-1))对菜青虫(Pieris rapae L.)和小菜蛾(Plutella xylostella L.)的选择性拒食率高达100.00%;对桃蚜[Myzus persicae (Sulzer)]和萝卜蚜(Lipaphis erysimi(Kahenbach)]的选择性忌避率分别为61.11%和70.83%;对小菜蛾的24 h选择性产卵忌避率达71.17%,并对小菜蛾幼虫的化蛹率和蛹重也有显著影响.经HPLC和LC-MS分析,组分Ⅱ的主要化学成分为倍半萜内酯类化合物4β,5β-环氧苍耳素-1α,4α-内过氧化...

Eight active fractions were separated from the ethyl acetate extracts of Xanthium sibiricum Patrin. leaf methanol extracts by silica gel column chromatography, and their effect of antifeedant and deterrent on four insect pests were investigated in laboratory. The results show that among the eight fractions, the biological activity of fraction II is the highest. The selective antifeedant rate of fraction II (0.01 g · mL-1) against Pieris rapae L. and Plutella xyiostella L. is 100. 00% , and its selective deterrent rate against Myzus persicae (S...