期刊论文

Quantitative Analysis of Tea Saponin in Camellia Cake

中文

精细化工中间体

1009-9212

2009

39

2

67-69

10.19342/j.cnki.issn.1009-9212.2009.02.022

本校为第一机构

研究了用反相高效液相色谱法(HPLC)测定茶皂素含量的方法,以VP-ODS(250 mmx4.6 mm,C18,5μm)作分析柱,以甲醇-水(v:v=9:1)作流动相,流速控制在1.0 mL/min,检测波长280 nm,以质量浓度为横坐标,峰面积为纵坐标,得到工作曲线为Y=3.02x106X-1.16x104,线性相关系数为0.999 5.回收率为97.02%～102.82%.通过反相高效液相色谱法测定茶籽饼中粗茶皂素的含量为11.89%.

A reverse-phase high performance liquid chromatography （HPLC） method for determination of tea saponin extracted from tea-seed pancake with Soxhlet was studied. With the column VP-ODS （250 mm×4.6 mm, C18, 5μm） and mobile phase methanol-water （v：v=9：1） at flow-rate of 1.0 mL/min, a linear equation was obtained as Y=3.02×10^6X-1.16×10^4, where Y represents peak area and X represents mass concentration. Linear correlation coefficient r was 0.999 5. Under these conditions, ...