期刊论文

Yang Zeng<&wdkj&>Dao-Hong Zhu

英文

Journal of Asia-Pacific Entomology

1226-8615

2021

24

2

30-34

10.1016/j.aspen.2021.04.007

A total of 30.79 GB clean data was obtained from the transcriptome analysis of four V. aspersus samples and the average clean data was 7.70 GB (Table 1). All clean data was assembled by Trinity (https://github.com/trinityrnaseq/trinityrnaseq/wiki ). The total number of unigenes and transcripts were 44,623 and 65507, respectively, and sequences with length between 0 and 500 bp accounted for the largest proportion (unigenes, 46%; transcripts, 43%) (Fig. 1). 6201 SSR-tagged sequences and 7878

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Microsatellite markers have been used extensively in studies of sperm competition and the genetic diversity of organisms. In the present study, we performed transcriptome sequencing for Velarifictorus aspersus and developed simple sequence repeat markers to identify valuable markers for genetic studies. The average total length of the V. aspersus transcriptome sequence was 7.70 Gb and it contained 7878 microsatellite loci. Sixty-four microsatellite loci were randomly selected for primer design and 39 loci were successfully amplified, among whic...