期刊论文

Screening, identification and physiological characteristics of an actinomycete strain against Rhizoctonia solani

Zhang, Q.-R.

中文

生态学杂志

Chinese Journal of Ecology

1000-4890

2014

33

2

394-399

10.13292/j.1000-4890.2014.0020

国家自然科学基金项目(40801202)； “十二五”国家科技支撑计划项目(2012BAC17B04)； 辽宁省自然科学基金项目(20102232)； 教育部留学回国人员科研启动基金项目资助；

本校为第一机构

通过对峙实验,从采集自黄渤海海域的双齿围沙蚕(Perinereis aibuhitensis)中分离到一株内生放线菌SCF-18,并对其抑菌活性进行分析。SCF-18对立枯丝核菌(Rhizoctonia)和小麦根腐病菌(Bipolaris sorokiniana)都具有拮抗作用,且对立枯丝核菌生长的拮抗效果最为明显,生长抑制率为89.78%。抑菌试验结果表明:菌株SCF-18发酵滤液可以抑制立枯丝核菌丝的生长,发酵液浓度越高,抑制力越强;当发酵液浓度达到50%时,对两种病原菌的抑制率分别为89.78%和80.26%。菌株SCF-18生长特性试验结果表明:菌株 SCF-18生长最适温度为30℃,最适pH值为7;放线菌SCF-18耐盐度可达5%。根据形态特征、培养特征、生理生化特性和...

By using dual culture technique, a strain of endophytic actinomycete named SCF-18 was screened out and isolated from Perinereis aibuhitensis collected from the Huanghai and Bohai Sea, and the bio-activities of the actinomyeete against pathogenic fungi were determined. The strain SCF-18 showed significantly antagonistic activities to both Rhizoctonia solani and Bipolaris sorokiniana, particularly more potent to R. solani with the maximum inhibition rate of 89.78%. The fermentation filtrate of SCF-18 could inhibit the growth of R. solani in a dos...