期刊论文

中文

食品与生物技术学报

1673-1689

2024

43

05

122-129

10.12441/spyswjs.20220929004

2019JJ40541:湖南省自然科学基金项目 20A513:湖南省教育厅重点项目

本校为第一机构

为探寻安全、高效的黄曲霉新型抑制物，采用顶空固相微萃取-气质联用(HS-SPME-GCMS)技术对枯草芽孢杆菌（Bacillus subtilis）PW2所产具有抗霉活性的挥发性有机化合物（volatile organic compounds, VOCs）进行了成分鉴定，并利用平板对扣法从鉴定出的成分中筛选具有强抗霉活性的目标化合物，通过平板对扣法和96孔板梯度稀释法测定目标化合物对黄曲霉的最小抑菌体积分数（MIC)，使用黄曲霉毒素B1(aflatoxin B1, AFB1)ELISA检测试剂盒分析了目标化合物对黄曲霉产AFB1的抑制作用，使用扫描电子显微镜(scanning electron microscope, SEM)观察受试黄曲霉孢子形态的变化和采用分光光度法测定受试黄曲...

In order to investigate safe and efficient novel inhibitors of Aspergillus flavus(A.fllavus),the compounds of the antifungal volatile organic compounds(VOCs)produced by Bacillus subtilis PW2 were identified by headspace solid-phase microextraction gas chromatography-mass spectrometer(HS-SPME-GC-MS)technique.The target compounds with strong antifungal activity were screened from the identified components by double petri dish method.The minimum inhibitory concentration(MIC)of the target compound against A.flavus was determined by double petri dis...