An antifungal Bacillus strain isolated from the traditional fermented food natto was identified and named as B. subtilis natto CSUF5. Antifungal metabolites of the strain were extracted from the medium of the inhibition zone on the dual culture plate. 2 V-7-surfactin isoforms (C-14-V-7, C-13-E-1(met) -V-7) and 3 I/L-7-surfactin isoforms (C-13-I/L-7, C-13,(14)-E-1(met)-I/L-7) were identified from the RP-HPLC isolations by MSn (n = 1,2,3) analysis. Both V-7-surfactins and I/L-7-surfactins showed slight antifungal activity against A. niger, and their MIC50 decreases in the order V-7 > I/L-7. EDTA...