With the enrichment of public databases, the release of Eucalyptus genome sequence data has gradually been increasing. In this study, 21 Eucalyptus chloroplast matK gene sequences were downloaded from the NCBI database for analytical use. Depending on the software used, we found the phylogenetic trees obtained could differ from the morphological taxonomic status according to Hill Johnson, which is accepted as the academic standard. These differences might relate to the sequence data not being comprehensive, as the amount of information obtained...