期刊论文

中文

化学研究与应用

1004-1656

2013

25

5

636-641

10.3969/j.issn.1004-1656.2013.05.005

本校为其他机构

采用超声波辅助半仿生法提取石榴皮单宁。利用正交实验方法对提取工艺进行优化,并进行了红外光谱分析和研究抗氧化能力检测。在提取液的pH值分别:1.2、7.6、8.4,溶剂质量分数50%、提取时间45 min、提取温度50℃、超声波功率320 W和超声波频率25KHz的条件下,单宁提取量可达234.6 mg.g-1,远高于无超声波辅助的试样。红外光谱分析表明:石榴皮单宁中含有大量的无色花翠素-3-葡萄糖苷分子结构式类物质;抗氧化能力检测显示:采用超声波辅助半仿生法能够提高单宁对OH.、DPPH.和ABTS+.等自由基的清除能力,与水回流提取法相比,其IC50值分别降低了0.26、0.022和0.41 mg.mL-1。

Tannin was extracted from pomegranate peel using the ultrasonic-assisted semi-bionic approach. The process was optimi- zated by orthogonal experiment method. The Infrared spectrum analysis and antioxidation capacity assay were adopted. The orthogo- hal experiment showed that the tannin extraction quantity can reach 234. 6 mg · g^-1, which was much higher than the sample obtained without ultrasonic assistance under the following experimental conditions ：pH values of 1.2,7.6, and 8.4, respectively ;50% solvent mass fraction;45min extraction tim...